Hard X-ray imaging of bactirial cells: nano-diffraction and ptychographic reconstrcuction. @article{osti_1756392, title = {Ptychographic atomic electron tomography: Towards three-dimensional imaging of individual light atoms in materials}, author = {Chang, Dillan J. and Kim, Dennis S. and Rana, Arjun and Tian, Xuezeng and Zhou, Jihan and Ercius, Peter and Miao, Jianwei}, abstractNote = {Through numerical simulations, we demonstrate the combination of ptychography and atomic electron . Phys. Ptychographic X-ray computed tomography (PXCT) is a potential tool for visualizing three-dimensional (3D) structures of large-volume samples at high spatial resolution. Ptychographic X-ray computed tomography (cSAXS beamline - Swiss Light Source, Paul Scherrer Institut, 90 K, 6.2 keV photon energy, 800 projections, 0-180° angle) d. 3D rendering images (2D reconstructed and processed slices were rendered using VG Studio MAX Software, Volume Graphics) e. Ptychographic x-ray tomography of integrated circuits in three dimensions with high resolution By Arthur L. Robinson April 21, 2017 Reconstructed image of the internal structure of a sample taken from a processor at the transistor level. Optics Express 20, 19232-19254 (2012). Ptychographic X-ray Tomography of Silk Fiber Hydration. Ptychographic tomography of nanocomposites The spatial organization of the worms and vesicles within individual calcite crystals were determined using cryo-ptychographic X-ray computed tomography (cryo-PXCT) at 180 C.35,37,42 These measurements were carried out by randomly selecting crystals that were 10-50 mm in diameter, Fourier ptychographic diffraction tomography 124,127 or multi-slice modelling 9,122,123 can be used to handle 3D specimens. [Crossref] Ptychographic X-ray tomography reveals additive zoning in nanocomposite single crystals . Nature 467, 436-440 (2010). Despite its unmatched reach, its application remains prerogative of a limited number of laboratories at synchrotron sources. Unlike the related method of diffraction tomography, FPT just measures normal intensity-only images, and does not need a reference beam to measure phase. Upper photo is the on- Studying noninvasively the internal nanoporous structure of a single Tussah silk fiber under different humidity conditions, we demonstrate for the first time the feasibility of in-situ ptychographic tomography. Fourier ptychographic diffraction tomography (FPDT) is a recently developed label-free computational microscopy technique that retrieves high-resolution and large-field three-dimensional (3D) tomograms by synthesizing a set of low-resolution intensity images obtained with a low numerical aperture (NA) objective. Nature 467 , 436-439 (2010). Phillips N, Putkunz C, Van Riessen G, Coughlan H, Jones M, Abbey B. Ptychographic Fresnel coherent diffraction tomography at the nanoscale. It has three key advantages: it does not . This paper presents a technique to image the complex index of refraction of a sample across three dimensions. . Here, we imaged, at cryogenic temperature and without sectioning, cellular and subcellular structures of a chemically fixed and stained wild-type mouse retina, including axons and synapses, with complete isotropic 3D information over tens of microns. Ptychographic tomography analysis of buried interfaces: submicron scale. A detector records an extensive data set consisting of many inference patterns obtained as an object is displaced to various positions relative to an illumination field. Then, using principles from ptychography and diffraction tomography, it computationally solves for the sample structure in three dimensions. S1 Supporting Information 4D Imaging of ZnO-Coated Nanoporous Al2O3 Aerogels by Chemically-Sensitive Ptychographic Tomography: Implications for Designer Catalysts Hao Yuan1, Hui Yuan2, Travis Casagrande2, David Shapiro3, Young-Sang Yu3, Bjoern Enders,4,5 Jonathan R. I. Lee6, Anthony van Buuren6, Monika M. Biener6, Stuart A. Gammon6, Theodore F. Baumann6 and Adam P. Hitchcock1* This comes at the cost of it being a scanning technique, meaning that acquisition time is generally long since it depends on the field of view that is covered. Clariant AG is acknowledged for the provision of samples and material characterization expertise. Whilst multi-slice ptychography can handle multiple-scattering thick specimens and has a much . M. Dierolf et al., " Ptychographic x-ray computed tomography at the nanoscale," Nature 467, 436- 439 (2010). Rev. We present here some Reference: Lei Tian, et al., Compressive X-ray phase tomography based on the transport of intensity equation, Opt. FP for education. fluorescence and ptychographic tomography to be a powerful tool for probing a wide range of frozen-hydrated biological specimens, ranging from small prokaryotes such as bacteria, algae, and parasites to large eukaryotes such as mammalian cells, with applications that include understanding cellular responses to environmental stimuli Here we report the first implementation of ptychographic X-ray computed tomography, which exploits the high phase sensitivity of two-dimensional (2D) coherent diffractive imaging to generate. Ptychography is a form of Coherent Diffractive Imaging, where diffraction patterns are processed by iterative algorithms to recover an image of a specimen. projections, ptychographic tomography can provide 3D quantitative measurements of electron density [11, 12]. To this end, we report a system calibration procedure, termed SC-FPM, to calibrate the mixed systematic errors simultaneously from an overall perspective, based on the simulated annealing algorithm, the LED intensity correction method, the nonlinear regression process, and the adaptive step-size strategy, which involves the evaluation of an . Nature 467 , 436-439 (2010). PHYSICAL REVIEW B102, 174101 (2020) Ptychographic atomic electron tomography: Towards three-dimensional imaging of individual light atoms in materials Dillan J. Chang , 1Dennis S. Kim ,1 Arjun Rana, 1Xuezeng Tian, Jihan Zhou, Peter Ercius ,2 and Jianwei Miao 1Department of Physics & Astronomy and California NanoSystems Institute, University of California, Los Angeles, California 90095, USA ADS CAS PubMed Google Scholar By solving the phase problem at different projection angles, ptychographic tomography retrieves the complex- The following figure displays the experimental set-up of the method. Ptychographic tomography relies on 2D ptychography to supply projections to conventional tomographic algorithms, whilst multi-slice ptychography uses the redundancy in ptychographic data to split the reconstruction into a series of axial slices. Nature 467, 436-439 (2010). We will concentrate on the analysis of the phase-contrast . All the data was acquired at the cSAXS beamline at the Paul Scherrer Institute, Switzerland. Although mostly applied in two. 12.66 keV vs 6.2 keV 38, 3418-3421 (2013). Ptychographic Fresnel coherent diffractive imaging. The 3D TV-minimization is implemented by a modified TwIST algorithm. She is responsible for user support at the beamline, and has developed ptychographic topography, a novel high-resolution strain imaging technique, as well as continuing her investigations of bone tissue by correlative study with small angle X-ray scattering tensor tomography and ptychographic tomography. Quantitative X-ray phase nanotomography. 3D Ptychographic Tomography 3D Ptychographic X-Ray Tomography is a scanning coherent X-ray diffractive imaging method which combines X-ray ptychography imaging together with 3D X-ray tomography technique. H … Ptychographic X-ray computed tomography is a phase-contrast imaging technique capable of retrieving three-dimensional maps of the index of refraction of the imaged volumes with nanometric resolution. Monitoring moisture distribution in textile materials using grating interferometry and ptychographic X-ray imaging. The concept of FP can help students understand the basics of Fourier optics, including coherent transfer function, NA, pupil aberration, Ewald sphere, etc. B 85, 020104(R) (2012). Dierolf, M. et al. Dierolf, M. et al. @article{osti_1762114, title = {4D Imaging of ZnO-Coated Nanoporous Al2O3 Aerogels by Chemically Sensitive Ptychographic Tomography: Implications for Designer Catalysts}, author = {Yuan, Hao and Yuan, Hui and Casagrande, Travis and Shapiro, David and Yu, Young-Sang and Enders, Bjoern and Lee, Jonathan R. I. and van Buuren, Anthony and Biener, Monika M. and Gammon, Stuart A. and Baumann . In case of the samples studied herein, the resolution of microtomography (micrometer scale) is not enough, but synchrotron tomography, more specifically, ptychographic X-ray computed tomography (PXCT), can image different structural features from the micrometer to the nanometer scale with high resolution, even in air at atmospheric pressure , . GENFIRE x-ray fluorescence and ptychographic tomography reconstructions. By Pierre Thibault. Our technique, termed Fourier ptychographic diffraction tomography (FPDT), uses a low-NA objective to acquire a sequence of intensity images corresponding to different illumination angles scanned sequentially with a programmable light-emitting-diode (LED) array. Vine D. J. et al. [PMC free article] Diaz A. et al. The combination with tomography makes 3D imaging possible via ptychographic X-ray computed tomography (PXCT), which can provide a 3D map of the complex-valued refractive index of the sample. Currently, both the requirement of a large number of views and the narrow depth of field limit the range of applications of PXCT. Ptychographic tomography instrumentation was supported by the Swiss National Science Foundation (SNF) (R′EQUIP, 145056, OMNY) and the Competence Centre for Materials Science and Technology (CCMX) of the ETH-Board, Switzerland. L. Tian and L. Waller, "3D intensity and phase imaging from light field measurements in an LED array microscope," Optica 2(2), 104 (2015). This aspect makes the technique especially attractive . A ptychographic reconstruction is achieved by means of solving a complex inverse problem that imposes constraints both on the acquisition and on the analysis of the . b, bragg. This method uses a ptychographic coherent imaging approach to record tomographic data sets, exploiting both the high penetration power of hard X-rays and the high sensitivity of lensless imaging. The extension of PXCT to X-ray energies above 15 keV is challenging, but it can allow the imaging of object opaque to lower energy. Lett. a, ptycho-tomography, where ptychography is combined with a highly precise rotation stage to achieve quantitative phase and amplitude reconstruction in 3d with few-nanometre resolution. Sun, J. Li, A. Asundi, and Q. Chen, "Wide-field high-resolution 3d microscopy with fourier ptychographic diffraction tomography," arXiv preprint arXiv:1904.09386 (2019). The only required hardware is a standard microscope and an array of LEDs. Multi-slice ptychographic tomography. Here ptychographic X-ray computed tomography is used to visualize the three-dimensional structures of two nanocomposite crystals - single crystals of calcite occluding diblock copolymer worms and vesicles. We demonstrated that ptychographic tomography is a promising technique that combines imaging of representative sample volumes with high resolution by combining coherent diffractive imaging and a scanning approach, thereby closing the resolution gap between electron microscopy and X-ray imaging techniques. Set-up of the 3D ptychographic X-ray tomography. Ptychographic X-ray computed tomography4 isbecominga lenslesscompletelyimaging technique in which the phase problem is solved by means of iterative phase retrieval algorithms. As a main structural level in colloidal food materials, extended colloidal networks are important for texture and rheology. Our recent work on cryo-EM ptychography (cryo-EP) demonstrated the application potential of the technique in characterising biological structure with dose-efficiency, signal-to-noise ratio, and large field-of-view. Ptychographic X-ray computed tomography at the nanoscale. ptychographic X-ray computed tomography. International Journal of Materials Research (formerly Zeitschrift fuer Metallkunde) Nicholas W. Phillips ( A ) 0° projection view of reconstructed 3D volumes of P (red), Ca (green), S (black), Cl (yellow), and K (blue) channels. The method, termed Fourier ptychographic tomography (FPT), first captures a sequence of intensity-only images of a sample under angularly varying illumination. In this article we address a nonlinear least squares model for the blind ptychography problem with constraints on the image and the probe by . Ptychographic hard X-ray computed tomography (PXCT) is a recent method allowing imaging with quantitative electron-density contrast. Ptychographic x-ray computed tomography (PXCT) is one of many methods for gaining access to three-dimensional internal information of a specimen. PLoS ONE 7, e35691 (2012). The strength and contrast of the phase signal also means that far fewer photon or electron counts are needed to make an image : this is very important in electron ptychography, where damage to the specimen is a major issue that must be avoided . Fourier ptychographic diffraction tomography (FPDT) is a recently developed label-free computational microscopy technique that retrieves high-resolution and large-field three-dimensional (3D) tomograms by synthesizing a set of low-resolution intensity images obtained with a low numerical aperture (NA) objective. Digital Holography The energies were chosen as: the optimal energy for the beamline (6.2 keV), just below the Se K edge (12.51 keV) and at the Se K edge (12.66 keV) respectively. The method, termed Fourier ptychographic tomography (FPT), first captures a sequence of intensity-only images of a sample under angularly varying illumination. As this method continues to advance with brighter coherent light sources and more efficient data handling, we expect correlative 3D x-ray fluorescence and ptychographic tomography to be a powerful tool for probing a wide range of frozen-hydrated biological specimens, ranging from small prokaryotes such as bacteria, algae, and parasites to large . We describe a coupled ptychography-tomography reconstruction algorithm and apply it to an experimental ptychographic x-ray computed tomography data set of a catalyst particle. Download. In ptychographic X-ray computed tomography (PXCT) experiment scanning translations are controlled with high-precision piezo-stages, while run-out errors of rotational stages are . X-ray Phase Nanotomography Resolves the 3D Human Bone Ultrastructure. The U.S. Department of Energy's Office of Scientific and Technical Information Bio of Angel Rodriguez-Fernandez Although tremendous progress in ptychography has been made in recent years, there have still been limitations due to coherent flux, radiation damage, and the speed of data acquisition and reconstruction. We call our new measurement and reconstruction process "Fourier ptychographic tomography" (FPT, for short). X-ray ptychographic computed tomography at 16 nm isotropic 3D resolution M. Holler 1, A. Diaz , M. Guizar-Sicairos1, P. Karvinen , Elina Fa¨rm2, Emma Ha¨rko¨nen 2, Mikko Ritala , A. Menzel 1, J . International Journal of Materials Research (formerly Zeitschrift fuer Metallkunde) Nicholas W. Phillips Related Papers. By A. Hipp and M. Bech. In terms of field of view, in-line phase tomography currently offers the largest, due to it . But in general, a drawback of tomographic techniques is the requirement for dense, time consuming angular scans. Dierolf M. et al. Cryo ptychographic X-ray computed tomography in the multi-keV energy range is an emerging technology providing phase contrast at high sensitivity, allowing label-free and non-destructive three dimensional imaging of large continuous volumes of tissue, currently spanning up to 400,000 μm 3. Here we report the first implementation of ptychographic X-ray computed tomography, which exploits the high phase sensitivity of two-dimensional (2D) coherent diffractive imaging to generate quantitative3Ddensitymapsofextendedspecimensonthenanoscale. Dierolf M. et al. C. Zuo, J. A computer algorithm of some type is then used to invert these data into an image. Ptychographic X-ray computed tomography at the nanoscale. The resulting 3D images of the silk fiber interior, obtained at both dry and humid conditions, yield quantitative information about the . Compared to the traditional sequential algorithm, in which ptychographic projections are reconstructed to . Wilke R. N. et al. International Journal of Materials Research . 1(c) and (d), respectively). We demonstrated that ptychographic tomography is a promising technique that combines imaging of representative sample volumes with high resolution by combining coherent diffractive imaging and a. Ptychography has rapidly grown in the fields of X-ray and electron imaging for its unprecedented ability to achieve nano or atomic scale resolution while simultaneously retrieving chemical or magnetic information from a sample. Although mostly applied in two dimensions, ptychography can be extended to produce three dimensional images in two ways: via multi-slice ptychography or . Ptychography is a form of Coherent Diffractive Imaging, where diffraction patterns are processed by iterative algorithms to recover an image of a specimen. PXCT provides a more detailed non-destructive analysis of the interface region, permitting to clearly distinguish several layered regions based on phase and absorption contrast (Fig. Ptychographic X-ray computed tomography at the nanoscale. OPEN X-ray ptychographic computed SUBJECT AREAS: tomography at 16 nm isotropic 3D TECHNIQUES AND INSTRUMENTATION resolution IMAGING TECHNIQUES M. Holler1, A. Diaz1, M. Guizar-Sicairos1, P. Karvinen1, Elina Fa ¨ rm2, Emma Ha ¨ rko¨nen2, Mikko Ritala2, A. Menzel , J. Raabe & O. Bunk 1 1 1 Received 29 November 2013 1 Paul Scherrer Institut, 5232 Villigen PSI, Switzerland, 2Department of . As this method continues to advance with brighter coherent light sources and more efficient data handling, we expect correlative 3D x-ray fluorescence and ptychographic tomography to be a powerful tool for probing a wide range of frozen-hydrated biological specimens, ranging from small prokaryotes such as bacteria, algae, and parasites to large . Ptychographic tomography is the technique that has permitted the highest resolution so far. A ptychographic reconstruction is normally formulated as a blind phase retrieval problem, where both the image (sample) and the probe (illumination) have to be recovered from phaseless measured data. Layers of interest . In the latter case, the phase signal is also ideal for high-resolution X-ray ptychographic tomography. To further realise the potential of the technique, we are developing a new workflow of cryo-EM ptychographic tomography (cryo-EPT) and cryo-EM ptychographic subtomogram averaging . 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