harvard microscopy core

Small Molecule Mass Spectrometry: mass spectrometry-based techniques to investigate a wide variety of analytes. These facilities provide scientists with access to highly specialized services, equipment, and expertise that cannot be supplied by an individual laboratory. The goal of the HMS Connectomics Core is to help researchers acquire, align, segment, proofread, and annotate large-scale maps of cell-cell interactions using volumetric electron microscopy (EM), genetically encoded cell-type-specific EM tags, trans-synaptic viral tracing, machine learning algorithms, and advanced . The core facility staff assists with technical problems, procedures, and training on instrumentation inclusive of; Cryostats, automated tissue processors, microtomes, and fluorescence and phase microscopes. The PMB houses an extensive Microscopy Core Facility that is an MGH-registered Core Facility, available on a fee-for-service basis. The Neurobiology Imaging Facility is a completely open access light microscopy and tissue core located within the Neurobiology Department. The Microscopy Core aims to provide powerful imaging tools to enable state-of-the-art research on neurological disorders and promote interdisciplinary collaboration. Core Director. All aspects of microscopy from basic wide-field and fluorescence imaging, confocal imaging, live cell imaging and TIRF (total internal reflection microscopy), and electron microscopy are available. Oct 2016 - Present4 years 6 months. The Deltavision was donated by the Cell Biology Department. 1880, agad@bidmc.harvard.edu Electron Microscopy EM Core Supervisor: Susan J. Hagen, PhD, (617) 667-4188 Confocal Specialist: Kyle Smith, (617) 667-4188; khsmith@bidmc.harvard.edu The Microscopy Core has two active services. Confocal Microscopy. For confocal, $65/hour for RIA division, $95 for other divisionss. Additionally, investigators have access to a number of . This technique provides visualization of the atomic lattice, providing important information about a materials structure, defects, homogeneity and its composition. The effort in all the facilities is to provide prompt, customized service. The Core performs routine histology and special histochemical stains and also assists in experimental design and the development, application, and interpretation of biomarker tests and their results. At CNS, we have four HRTEMs available for materials analysis. Assistant Professor of Stem Cell and Regenerative Biology, Harvard University. Advanced Imaging Specialist. The Enhanced Neuroimaging Core is a fee-for-service core that provides access to advanced confocal, multi-photon and wide field microscopy services throughout the NeuroDiscovery community. Core Director - Maria Ericsson Core Website - http://electron-microscopy.hms.harvard.edu/ CryoEM Center The Confocal Core Facility now services researchers in BWH only. Training: Faculty, staff, postdoctoral fellows, and students who wish to use the Zeiss LSM880-Airyscan confocal laser system in the BCH-HDDC microscopy core facility should register for an account on iLAB to enable training and scheduling. Email. Bio. Director: Seth Goldman, PhD. The Morphology Core laboratory is located on the seventh floor of Harvard's New Research Building (HNRB), within the Center for Excellence in Vascular Biology's Vascular Research Division of Brigham & Women's Hospital. The Cell Biology Microscopy Facility (CBMF) provides the Harvard Medical School community with advanced optical microscopy expertise and equipment. MicRoN is a non-traditional decentralized or "floating" microscopy core. The MicRoN core (Microscopy Resources on the North Quad) is a light microscopy core at Harvard Medical School that serves ~75 different groups throughout the Longwood Medical Area. Research Assistant (TEM/CLEM) Tel: 617 432 1693. The MicRoN core (Microscopy Resources On the North Quad) is a light microscopy core at Harvard Medical School that serves ~75 different groups throughout the Longwood Medical Area. Welcome to Brigham and Women's Hospital Confocal Microscopy Core website, located within the Division of Rheumatology, Inflammation and Immunity. This is a new option for HDDC members for which the methods are currently in development (2021). Dry Ice. Our facility is equipped with one of the most advanced, and yet highly user-friendly, confocal system, Zeiss LSM 800 with Airyscan. The purpose of the Animal Physiology Core is to provide technically advanced physiological evaluation of metabolism in diabetes, obesity, and the associated complications in rodents for Joslin Diabetes Research Center investigators and outside users. The Harvard Medical School EM Facility is a fee-for-service core facility open to researchers in the HMS Quadrangle and the Longwood Medical area. Description: This course will focus on advanced quantitative fluorescence microscopy techniques used for imaging a range of biological specimens, from cells to single molecules. The complexity of the human brain exceeds that of any observable system in the known universe. Reserve time on the systems using the ONLINE SCHEDULER.If you would like to become a registered user, please contact Steve Turney (sturney@mcb.harvard.edu).The imaging facility comprises seven microscope systems: three Zeiss upright confocal microscope systems . Use of the DeltaVision is restricted to members of labs whose principal investigator has a faculty appointment in the Department of Cell Biology. The Neurobiology Imaging Facility is a completely open access light microscopy and tissue core located within the Neurobiology Department. The Facility provides services and supervision in Transmission and Scanning Electron Microscopy. Core Facilities | Harvard Stem Cell and Regenerative Biology. Histology Core at HSCRB | HSCRB Our Histology Core provides laboratories across Boston with an array of histological and immuno-histochemical services. Greater Boston Area. Bio-Rad CFX Connect Quantitative PCR machine. Core Services. To book, please contact Kelly Horn, khorn2@usf.edu to discuss using this core facility. We offer both full-service and self-service options. Immunoelectron microscopy; Facilities. Histology(paraffin and frozen sections) . PLEASE VERIFY ALL RUSH WORK REQUESTS WITH US BEFORE PLACING THEM ON OUR WEBSITE. * Harvard Internal rates = users with Harvard 33-digit billing codes. Personnel/Contact Information: Core Manager & EM Specialist: Philip Seifert Phone: (617) 912-0209 Email: philip_seifert@meei.harvard.edu. sea urchin sperm, Rick Ng, Megan Parsons, Ellery Jones/MCB 68. Praju did his Ph.D. Singapore where he worked on cancer metastasis and the actomyosin network. The Confo-cal Microscopy and Immunostaining Service provides technical assistance and instrumentation for immunos-taining . 16 Center for Cell Imaging, University of Liverpool, Liverpool, UK. Situated in the Bauer Building (room B008) at 7 Divinity Ave., Cambridge, we offer services to any laboratory requiring histology support. The Facility provides services and supervision in Transmission Electron Microscopy. Optical Imaging Facility: The K-FLARE open air NIR imaging system was designed by Dr. John Frangioni and engineers at BIDMC. MIC was recently re-organized under the faculty Co-Directors, Kai Wucherpfennig, MD, PhD, and Judith Agudo, PhD with the focus on meeting imaging needs for basic scientists. During the course of his doctoral research, Chen co-invented expansion microscopy, a breakthrough technique that allows for super-resolution imaging of biological samples with conventional light . Optical Imaging Core. Harvard University CNS is an "Open" Core Research Laboratory devoted to the Support of Transformative Nanoscience. The Harvard Medical School EM Facility is a fee-for-service core facility open to researchers in the HMS Quadrangle and the Longwood Medical area. drosophila neuro-muscular junction, Chloe Warinner Jumai Yusuf/MCB 68. Our mission is to advance research by providing services in optical imaging, tissue clearing, in situ hybridization as well as introduce new equipment and cutting technology to the basic . Center for Brain Science: neuroimaging, light microscopy, and more. The Microscopy Resources On the North Quad (MicRoN) facility is a light microscopy core established by the Departments of Genetics, Immunology and Microbiology, and it is open to all HMS researchers, including Affiliated hospitals. Services that the Core provides focus mainly on mouse histopathology, and include necropsy, phenotyping of organs and tissues, dissection and tissue processing, and one-on-one consultation. Email questions to DGLUHOVAKASSIS@mgh.harvard.edu. We are located on the Harvard Medical School campus in the basement . Specific services include immunohistochemistry, immunofluorescent staining, in situ hybridization, and access to Fluorescence Microscope. The German Society for Microscopy and Image Analysis, known as German BioImaging, has offered a course on core-facility management and leadership in Germany since 2013, and Global BioImaging, an . Overview. The core will be utilizing software (TeamViewer) that allows the researchers to participate in their imaging session remotely. Email: brown.dennis@mgh.harvard.edu. The Facility provides services and supervision in Transmission Electron Microscopy. Core Summary: The Neurobiology Imaging Facility is the Neurobiology Department core facility for light microscopy. It is composed of an anti-vibration table and a black anodized stage contained within a light tight enclosure. Core Manager Microscopy, Boston Children's Hospital, Program in Cellular and Molecular Medicine @ Harvard Medical School Boston, Massachusetts 24 connections Join to Connect Core Member, Broad Institute of Harvard and MIT. Minimum reservation time is 30 min. 617-309-2660. In order to ensure that our researchers have access to cutting-edge tools, technologies and methodologies, Harvard University invests in shared core facilities from macro and micro-scale imaging technologies, genetics and behavioral . All systems will be replaced every 3 years and adapted to the needs of our community over time. Each Cs nanowire, several hundreds of angstroms long, is aligned along the [11̄0] direction. 212 of the Goldenson Building and is supported by a Core Grant (#P30 EY12196) from the National Eye Institute.If you have questions or comments, please contact Rick Born at the address at left. Eppendorf 5810R centrifuge. Harvard Center for Biological Imaging (HCBI) Microscopes will be available on a fee-for-service basis only. F 617-975-5562. Time is billed in from the start time of the reservation, until . ** Harvard affiliate rates = users without a 33-digit code, who pay by PO, with "Harvard" in their email address. Within the enclosure are fiber optic bundles for subject illumination, and attached to . Center for Nanoscale Systems: nanofabrication, materials synthesis, imaging, and computer analysis of design of nanoscale systems. 10/hr. HMS Connectomics Core. Given the intricate spatiotemporal regulation of the actomyosin components, his work inevitably involved a large microscopy element. Overview: Core B utilizes expertise in transmission electron microscopy to offer techniques, equipment, and expertise in focused ion beam (FIB)/ scanning electron microscopy (SEM) for super-resolution 3-D imaging of biological samples. For training, or for advice on choosing the best microscope for your application, please review our access policy and submit a request for training. About. Fei Chen, PhD. About The Core. Histology Technician: Bianai Fan Phone: (617) 912-0209 All training and unassisted use on all core shared equipment [embedder, paraffin microtome, cryostat, and microscope] will now be offered with limited capacity (1-2 people max per session). He promptly fell in love with imaging in . With our primary focus on Nanofabrication, Imaging and Nanoscale analysis, we at a "one stop shop" for all things nano. Services include light and epifluorescence microscopy . The Optical Imaging Core is a satellite of the FAS Division of Science's Harvard Center for Biological Imaging (HCBI) and utilizes the HCBI's unique lease-based procurement model. Major Equipment: Beckman Coulter Avanti J-E centrifuge. High Resolution TEM. Fax: 617-432-1639. margaret_coughlin@hms.harvard.edu. This page will serve as a general resource for users of the quantitative microscopy core in the Neurobiology Department at Harvard Medical School.This facility is housed in Rm. Confocal Microscopy. Peg Coughlin. The microscope systems in the imaging facility are made freely available to members of CBS , MCB and other departments. Connections Between Mouse Retina & Thalamus. Electron Microscopy (EM) Core. Kyoungmin Park. MicRoN is a light microscopy core that serves ~70 groups in the Longwood Medical Area. kaleidocell, Karagen Hudson Audrey Chen/MCB 68. Electron Microscopy. For training and access, contact: cryoem@crystal.harvard.edu or 617-432-7121. Brigham and Women's Hospital provides its scientific investigators with the tools they need to move research forward. Learn more about the Biobank. Histology Technician: Bianai Fan Phone: (617) 912-0209 Consultation for various histology, electron microscopy and microscopy procedures and troubleshooting, and training on all microscopes. 15/hr. The mission of the NTC is to offer the community a resource for the analysis of nascent transcription, providing new insights into gene regulation and enabling highly sensitive identification of regulatory . Gryzmish B005, 330 Brookline Avenue. BPF NGS GENOMICS CORE FACILITY BPF Genetics HMS^Biopolymers Facility-BPF (104585) Ciulla Ashley aciulla@genome.med.harvard.edu CELL BIOLOGY MICROSCOPY FACILITY Cell Bio Microscopy Cell Biology HMS^Nikon Imaging Center (104582) Waters Jennifer jennifer_waters@hms.harvard.edu Harvard Center for Biological Imaging. Grazing incidence monochromatic synchrotron X-ray topography, which is a lattice defect observational technique, has been used in power devices made from 4H-SiC. Sample Drop-off is 9AM-12PM. For questions, please call 1-888-802-4453 or email BCHBiobank4Discovery@childrens.harvard.edu. Biomedical CORE B: Microscopy and Histopathology. The PMB houses an extensive Microscopy Core Facility that is an MGH-registered Core Facility, available on a fee-for-service basis. Mapping axonal arbors with a neuronal positioning system (NPS) Room 2052 Biological Labs. Our mission is to advance research by providing services in optical imaging, introducing new optical imaging equipment, providing learning opportunities to researchers in the Longwood area and increasing the general imaging expertise of neuroscientists. The Rodent Histopathology Core is a Dana Farber/Harvard Cancer Center facility that is based at HMS. High-resolution transmission electron microscopy was used to analyze basal-plane dislocations, which display very characteristic contrasts in grazing incidence monochromatic X-ray topographic images, on the Si-face of 4H-SiC. Praju Vikas Anekal, PhD. The Advanced Microscopy Core provides a wide range of service covering the broad categories: performing specific morphological procedures, providing access to equipment and reagents, and giving advice, training and interpretation. Our services include slide scanning, confocal microscopy, lightsheet microscopy, multispectral imaging, live-cell imaging, super-resolution imaging, and imaging flow cytometry. Super-Resolution Sturctured Illumination. Core Facilities. Key Services The Microscopy Core Facility provides experimental consultation, fee-for-service imaging assistance, and training in and access to several imaging platforms, image acquisition equipment and data analysis software packages. 3.74/pound. The Molecular Biology and Genomics Core offers a suite of services in one location. NRB 1030. We present a combined scanning tunneling microscopy and ultraviolet photoelectron spectroscopy investigation on the formation and evolution of one-dimensional (1D) Cs chains on the InAs(110) surface. Cores and Resources for Investigators. The Harvard Catalyst Clinical Research Center at MIT . The core is in the process of merging with Neurobiology Imaging Facility. We were the very first to provide MP-IVM service to Longwood area scientists since that time, with expertise training relevant to the experiment being pursued. Dr. Bradlee Heckmann serves as the managing faculty adviser of the Byrd Microscopy Core. Multiple samples during the same day will result in . Processing workstations for image analysis and data processing will continue to be accessible remotely. kyoungmin.park@joslin.harvard.edu. Please remember to acknowledge the Harvard Digestive Disease Center and NIH grant P30DK034854 in all publications and presentations emerging from HDDC core services, resources, technology, and expertise. The Experimental Therapeutics (ETx) Core is a preclinical resource to Dana-Farber Cancer Institute (DFCI) researchers and collaborators for conducting in vivo pharmacological studies. At 40% of saturation coverage, the linear phase evolves into a two-dimensional (2D) cluster layer. The Harvard Stem Cell Institute's iPS Cell Core Facility provides investigators with customized iPS derivation and genome editing based on the latest cutting-edge stem cell technologies. P 617-667-4188. Boston, MA 02215. D. Phone: (617) 726-5665. Fei Chen is a core institute member at the Broad Institute of MIT and Harvard and an assistant professor in the Department of Stem Cell and Regenerative Biology at Harvard University. *** This rate is for one sample per day of equipment use. The Facility is open Monday-Friday 9AM - 5:30 PM. The Harvard Center for Cryo-Electron Microscopy (HC2EM) is a joint effort by Harvard Medical School, Dana-Farber Cancer Institute, Boston Children's Hospital, and Massachusetts General Hospital to provide state-of-the-art cryo-EM instrumentation and expertise for the Harvard structural biology community. Beth Israel Deaconess Medical Center. Studies will be run at a 14,000-square-foot preclinical facility shared with the Lurie Family Imaging Center (LFIC) that features bioluminescent imaging using the . All aspects of microscopy from basic wide-field and fluorescence imaging, confocal imaging, live cell imaging and TIRF (total internal reflection microscopy), and electron microscopy are available. Home | Electron Microscopy Facility at Harvard Medical School The Harvard Medical School EM Facility is a fee-for-service core facility open to all researchers. The core currently hosts 14 instruments including widefield, TIRF, spinning disc confocal, scanning confocal, multiphoton and super-resolution SIM-TIRF. High resolution transmission electron microscopy (HRTEM) is a powerful tool for materials analysis. Facilities and Equipment: Location of Core: 3 Blackfan Circle, Center For Life Science Building, Room13060, Boston, MA, 02115. The core currently hosts 14 instruments including widefield, TIRF, spinning disc confocal, scanning confocal, multiphoton, and super-resolution SIM-TIRF. The Ragon Microscopy Core provides cutting-edge imaging technologies to answer fundamental questions in immunology and infectious disease research. Live cell imaging is charged at the same rate. 15 UNC Neuroscience Microscopy Core Facility, Carolina Institute for Developmental Disabilities, and UNC Neuroscience Center, University of North Carolina, Chapel Hill, NC, USA. The Core Facility provides a unique and important resource for members of the scientific community as they approach a variety of research problems that call for these advanced microscopy techniques. Located in the Longwood Center Basement (LC-B1055), the Molecular Imaging Core (MIC) provides light microscopy imaging services that meet your imaging needs. The Microscopy Core of the Program in Membrane Biology (PMB) at MGH is equipped and staffed to provide a wide range of services to investigators from MGH and the Boston area scientific community in the area of light and electron microscopy. The course is designed for cell and molecular biologists with little or no microscopy experience, who wish to begin utilizing microscopy in their own research. Jeff Lichtman, Jeremy R. Knowles Professor of MCB, is studying the brain with the help of a larval zebrafish >>. Phone: (617) 919-2353. Other Core Facilities. It currently . During the course of his doctoral research, Chen co-invented Expansion Microscopy, a breakthrough technique that allows for super-resolution imaging of biological samples with conventional light For further information and contacts please click here. HSCRB core facilities support scientific researchers both within Harvard and across the Boston area. Fax: (617) 643-3182. Services Next Generation Sequencing (NGS): Illumina MiSeq Next Generation Sequencing system is the latest standard and can process one sample within 3 hours with 95% of samples delivered the same day allowing researchers . The core consists of two sites: The core offers three non-integrating technologies (Sendai virus, miRNA and modified mRNA, and episomal vector) for reprogramming of a variety of somatic cell . Our mission is to advance research by providing services in optical imaging, tissue clearing, in situ hybridization as well as introduce new equipment and cutting technology to the basic science community. Personnel/Contact Information: Core Manager & EM Specialist: Philip Seifert Phone: (617) 912-0209 Email: philip_seifert@meei.harvard.edu. Nascent Transcriptomics Core. 2.49/pound. . Including superresolution and lattice light sheet microscopy. MethodsJ2 automatically gathers metadata from the image using OME BioFormats (for example, pixel size, magnification) and captures microscopy metadata from a Microscope.JSON file generated using . Many of these technologies can be used in combination to facilitate synergistic projects. Email. 2020 USER AGREEMENT FOR THE LSM880 WITH FAST AIRYSCAN CONFOCAL SYSTEM. A variety of resources - both services and educational opportunities - are available to members of the BWH research community. Flow Cytometry and Microscopy Services The Immunology Core provides the Harvard University AIDS research community access to and training in leading-edge technology, techniques and assays addressing questions relevant to immunology and infectious diseases. Electron Microscopy image showing neural circuitry in the visual thalamus of a mouse>>. Consultation for various histology, electron microscopy and microscopy procedures and troubleshooting, and training on all microscopes. We operate a dedicated Multiphoton-Intravital Microscopy (MP-IVM) Facility, since the year 2000, on the second floor of the Warren Alpert Building at the Harvard Quad. The DeltaVision is an efficient wide field microscope with an environmental chamber providing heat and CO2, suitable for long-term live cell imaging For a list view, or to see archived configurations and microscopes, see our Microscope Listing. In concert with the Precision Link team, the core supports identification of the right cohort for a study, sample and data collection, and IT support. iLab: The BCH Imaging Core uses the iLab system for registering, training and scheduling. The Microscopy Core provides training on two Leica confocal microscopes and Nikon Epifluorescent Microscopes located at SERI-MEE. Director: Kenneth D. Mandl, MD, MPH. For widefield, $40/hour for RIA division, $55 for other divisions. Personnel/Contact Information: Director: Dennis Brown, Ph. Click on a category to sort our microscopes by imaging technique. 17 Microscopy Resources of the North Quad, University of Harvard Medical School, Boston, MA, USA. The Harvard Cryo-Electron Microscopy Center for Structural Biology (HC2EM) is a joint effort by Harvard Medical School, Dana-Farber Cancer Institute, Boston Children's Hospital, and Massachusetts General Hospital to provide state-of-the-art cryo-EM instrumentation and expertise for the Harvard structural biology community. Harvard Medical School. Bchbiobank4Discovery @ childrens.harvard.edu long, is aligned along the [ 11̄0 ] direction investigators have access to Microscope... 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